The traceability of product sourcing and production processes has become a crucial need for the Agri-food Industry, where stakeholders – particularly consumers – increasingly demand guarantees on product quality and authenticity. Blockchain technology, given its characteristics of immutability and transparency, can play a key role in solving issues related to traceability and information asymmetry, which undermine security and stakeholder trust in companies, as well as in promoting corporate social sustainability. The present study aims to fill a gap in literature by focusing on the operational application of this technology, with particular attention to the issue of credibility of the raw data entered into the system. To this end, a qualitative approach is adopted based on a multiple case study conducted through secondary data, integrated with primary data collected through a focus group. The work has a twofold objective: on the one hand, to explore the motives and goals that drive companies to implement Blockchain technology; on the other hand, to identify the preliminary conditions that ensure its operational effectiveness and credibility in the eyes of stakeholders. The results suggest that traceability is the main reason why companies decide to implement Blockchain technology, with the goal of ensuring product authenticity and transparency to the consumer. In addition, two sets of factors – operational and validation – were identified as key preconditions for effectively implementing a credible Blockchain information system. The study contributes to a deeper understanding of the operational application of Blockchain in the agri-food sector, providing relevant theoretical and practical implications.