This paper aims to introduce and validate a comprehensive toolkit tailored to assist cultural tourism organizations in managing digital transformation (DT) and business model innovation (BMI). As the cultural tourism industry faces the dual challenges of maintaining traditional values while integrating contemporary digital practices, this toolkit provides a strategic framework to navigate these complexities effectively. A mixed-method approach was adopted to develop and refine the toolkit, incorporating literature review, expert panel consultations, and pilot testing within various cultural tourism organizations. The toolkit includes four distinct tools: the Cultural Tourism Digital Maturity Assessment Tool, Digital Leadership Assessment Tool, DT SWOT Analysis Tool, and Business Model Innovation Profiling (BMIP) Tool, each designed to address different aspects of DT and BMI. The findings indicate that the toolkit is effective in helping organizations assess their current digital maturity, identify leadership capabilities, analyze strategic positions, and innovate business models. Feedback from pilot implementations suggests that the tools are practical and beneficial in guiding organizations through the intricacies of digital adaptation and strategic innovation. This research contributes uniquely to the field by offering a sector-specific toolkit that combines theories of digital transformation with practical, actionable strategies tailored for cultural tourism. It fills a significant gap in existing literature by providing a holistic, structured approach to DT and BMI specifically designed for the cultural tourism sector. The toolkit offers cultural tourism managers and decision-makers a practical and strategic resource to enhance their organization’s competitiveness and sustainability in the digital era. It facilitates a deeper understanding of internal and external digital dynamics and provides a clear pathway for strategic digital and business model innovation.