In the era of Industry 5.0, artificial intelligence offers new opportunities for co-creation, personalization, collaboration and integration. These affect companies’ Business Model Innovation strategies, supporting progress in the interactions between sets of actors, populations, activities, institutions and networks impacting the entire innovation ecosystems. Positioning itself in the research stream focused on analysing the impact generated by emerging technologies in the innovation of business ecosystems, this study aims of discussing the impact generated by artificial intelligence in business model innovation, reflecting on the salience of the involved stakeholders. A three-phase methodological approach was used, integrating a systematic literature review with a case study analysis. The related findings were discussed according the stakeholder theory basing on the salience attributes of power, legitimacy and urgency. This approach allowed us to establish a strong theoretical foundation while anchoring our findings in real-world example. Seven artificial intelligence-based value creation innovations were identified, demonstrating how artificial intelligence-based applications are transforming value creation mechanisms. Findings indicate that artificial intelligence has the potential to generate significant added value, primarily by enhancing value creation through data exploitation and the high degree of services customization tailored to customer needs. Moreover, new value streams emerge from artificial intelligence-based applications, driven by the widespread adoption of the technology across innovation ecosystems. This diffusion influences and engages stakeholders at every layer of the ecosystem’s structure. Stakeholders are classified as Discretionary (Standards Bodies, Public Bodies), Demanding (Distribution Channels, Research Institutes), Dangerous (other suppliers), Dependent (Suppliers), Definitive (Core Organization, Customers, Complementors). Notably, no stakeholders were identified that align with the Dormant or Dominant categories.