Knowledge and innovation are the main strategic keys for creation of new sustainable value of organizations (Dankbaar, 2003; Hayter, 2016). In according to Pacheco et al. (2014), the introduction of new knowledge is a driver for smart and sustainable growth in economic sector. The main producers and disseminators of new knowledge are Universities and Higher Educational Institutions (HEIs). Moreover, the university is opening up towards the entrepreneurial system. The main effect of this strategic orientation of University is the creation of collaborative ecosystems oriented to Knowledge Transfer (KT). This collaborative ecosystem opens reflections, for management and organization studies scholars, on the process of knowledge transfer between universities and firms. In effect, the introduction of new knowledge in traditional sectors, such as agri-business, shows more effects and are more problematic than other sectors. Agri-food organizations are based on consolidated managerial (and knowledge) practices and a low propensity for innovation. The Agri-food sector requires new practices that increase the competitiveness of firms. In particular, a growing area of research is focusing on the study of the academic knowledge transfer process and on the Key Organizational Factors (KOFs) that influence the KT process. For this reason, the present study intends to analyse the KT mechanisms (with particular focus on academic spin-offs) and KFOs with the aim of understanding the dynamics ecosystem for sustainable value in wine sector. This study is based on an exploratory and descriptive study. In particular, the first objective of this research is to understand the main theories on knowledge transfer phenomenon between university and agri-business firms. In this phase, we analysed some scientific databases (Scopus, Science Direct and Google Scholar). The second phase uses the interview method as a data collection tool on a sample of companies in the agri-food cluster. The study offers a mapping of main theoretical framework on Academic Knowledge Transfer in agri-business. Furthermore, it provides reflections on knowledge transfer mechanisms between universities in the agri-business sector. The results of this research offer an overview regarding main theories of knowledge transfer and effects of the third mission in agri-food sector. The study can help universities to manage KT’s mechanisms more effectively with the aim of generating new business organizations