In modern industrial economies, start-up companies are taking an increasingly central role in several government initiatives as a basic engine for socioeconomic development. The scientific literature shows that the success of these business initiatives depends on several factors. In addition to the intrinsic quality of each initiative, several researchers agree in the importance of factors related to the context in which start-ups live and operate. This study aims to analyze motivations, obstacles and success factors of start-up companies in Italy. First, we carried out a systematic literature review on start-up companies domain. In order to answer to the above-mentioned research questions, we collected data through an empirical study based on semi-structured interviews. Survey was conducted in June 2015. The questionnaire was completed by 348 start-up companies, with a response rate of 21,19%. Data were analysed through quantitative statistical techniques, such as factor analysis and ANOVA, to identify goals and critical success factors for Italian Start-uppers. Empirical studies revealed the presence of several regions on a global scale, characterized by the presence of successful start-ups. The presence of geographical areas globally acknowledged as real technological hubs (e.g. Silicon Valley) is due to the presence of a set of conditions that, in the system, are extremely favorable to their development. Although many researches provide frameworks to analyze start-ups ecosystems at a glance, we highlight that literature lacks of an analysis of Start-uppers profiles and goals, as well an identification of the critical success factors, according the Start-uppers’ perspective. Moreover, although there is already some significant body of knowledge on Italian Start-ups Panorama, many of these works are in the form of journalistic and non-academic books and reports. In Europe, too many promising high tech start-ups do not manage to grow and scale up at international level. Italy is characterized by a constellation of small businesses that, despite their peculiarities, have great difficulty in competing in the global market. Although the start-up phenomenon is growing, the Italian situation is still in its infancy, with many start-up with a small number of employees and still in early stage. The identification of the characteristics of the Italian start-up companies will allow a better understanding of this entrepreneurial phenomenon, supporting the design of appropriate innovation policies and regional industry development.