The livestock sector faces significant challenges that hinder innovation and the adoption of inclusive and sustainable practices, caused by its predominantly male-dominated structure and dependence on traditional methods. Women, while showing interest in sustainability and collaboration, face social and cultural barriers that prevent their entry and recognition as capable and independent agricultural entrepreneurs. Empowerment initiatives are essential to address these challenges, transforming women from powerless subjects to active participants in value creation. While recent literature explores the empowerment of women in agriculture, few studies comprehensively examine the mechanisms for promoting equality and empowerment and the benefits women could bring to the industry. This paper fills this gap by analysing the context and strategies of women’s collaboration and empowerment in the livestock sector, focusing on a case study of an Italian association that leverages a multi-stakeholder approach. The findings reveal the association’s role in promoting collaboration, knowledge sharing, and accessibility to resources and highlight the importance of addressing women’s cultural, social, financial and technical barriers and emphasise the value of collaboration and support networks. Recommendations for stakeholders include fostering partnerships, leadership training, and promoting open dialogue to recognize women’s contributions. However, the limitations of the study, including the focus on a single case, suggest the need for further research to generalise the findings and explore applicability in different contexts. Overall, this study contributes to the understanding of women’s empowerment in agriculture and offers practical insights to promote inclusivity and ageing in the livestock sector.