Observing the contribution that an approach using the Innovation Networks Analysis involving the Triple Helix spectrum, its contributions to the diffusion of a knowledge-based economy, potentializing regional socioeconomic development, the present study carried out a literature systematic review with the purpose to verify the contributions that Innovation Network Analysis, as a strategic analysis tool, can bring to establish strategic Regional Knowledge-Based Development. At the conclusion of this study, it was verified that Network Analysis and Knowledge-Based Development are emerging themes, with emphasis on scientific production in the last five years, and converge towards a strategic approach to policies and practices at local, regional, national and multinational levels, contributing to the diffusion of a knowledge-based economy, enhancing regional socioeconomic development.