The aim of this study is to explore the main barriers to the implementation and operation of knowledge management (KM) initiatives in public sector organisations in Chile, through the description of the case of a public sector organisation (OX), dedicated to scientific and technological development in a specific field. Through this case, it is possible to understand how difficult it can be to initiate a KM project in the specific context of this country due to the prevailing organisational culture, the lack of political and economic certainty regarding these initiatives and the abundant internal and external political activity of the organisation. In turn, this research highlights the importance of leadership, communication and the search for inventive and culturally adapted solutions to move projects forward. The main contribution of this research is to describe in more detail the complexities faced by KM teams in developing countries, especially in the public sector.