The purpose of this research is to highlight the need for effective knowledge exchange in intra-organisational contexts in relation to promoting innovation in the development and implementation of regional cultural strategies. This paper presents the first part of an ongoing comparative research project. The first case study (presented here) is based on Aberdeen City Council, the local government authority covering the City of Aberdeen in Scotland. The need for this research is driven by an acknowledgement of the relatively isolated positioning of ‘culture’ within public sector bodies and the potential impact of this position on the wider cultural sector. The research employed a comparative case study approach to highlight comparison between the case study organisations, and to develop an understanding of the impacts of those comparative factors. A review of the relevant literature was conducted to refine the research questions and identify gaps in the literature. A purposive sample of employees was invited to participate in a survey questionnaire. Secondary literature produced by employees for internal and external use was also consulted to enrich the primary data. Primary and secondary data was thematically analysed to generate relevant findings. The research shows that while there is the relevant expertise, a recognised need and genuine desire to exchange knowledge within the organisation in support of the development and implementation of cultural strategies, these are hampered by a range of factors including: the perceived value of the concept of culture; the value and support for cultural initiatives; and the development and maintenance of meaningful relationships. These factors impact on the production of innovative cultural strategies, and concomitantly on the effectiveness and sustainability of regional arts and cultural sector initiatives and organisations, as well as the individuals therein. It is anticipated that this research will be of interest to practitioners in cultural strategy development. In addition to highlighting barriers and enablers of knowledge exchange for cultural strategy development, the research also emphasises the need for a more rigorous attempts to define the culture of concept is contextually meaningful ways, to emphasise the value of culture at regional levels, and to develop a lexicon for cultural activities which can be used in multi-partner initiatives.